Composite

Part:BBa_K3747140

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-21)


nosRZDFYLTatA Pseudomonas stutzeri JM300

Nos is a nitrous oxide reductase, which is required for denitrification [1]. Nos is the last enzyme in the denitrification pathway and reduces nitrous oxide (N2O) into dinitrogen gas (N2). We expressed this construct in Pseudomonas putida EM42 together with a plasmid containing possible electron donors for Nos (BBa_K3747141)to establish denitrification in this organism.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2850
    Illegal PstI site found at 3150
    Illegal PstI site found at 3420
    Illegal PstI site found at 4557
    Illegal PstI site found at 4641
    Illegal PstI site found at 5864
    Illegal PstI site found at 7416
    Illegal PstI site found at 7875
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2850
    Illegal NheI site found at 559
    Illegal NheI site found at 3360
    Illegal PstI site found at 3150
    Illegal PstI site found at 3420
    Illegal PstI site found at 4557
    Illegal PstI site found at 4641
    Illegal PstI site found at 5864
    Illegal PstI site found at 7416
    Illegal PstI site found at 7875
    Illegal NotI site found at 8316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2850
    Illegal BglII site found at 1289
    Illegal BglII site found at 3790
    Illegal BglII site found at 5536
    Illegal XhoI site found at 2154
    Illegal XhoI site found at 2919
    Illegal XhoI site found at 6433
    Illegal XhoI site found at 7596
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2850
    Illegal PstI site found at 3150
    Illegal PstI site found at 3420
    Illegal PstI site found at 4557
    Illegal PstI site found at 4641
    Illegal PstI site found at 5864
    Illegal PstI site found at 7416
    Illegal PstI site found at 7875
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2850
    Illegal PstI site found at 3150
    Illegal PstI site found at 3420
    Illegal PstI site found at 4557
    Illegal PstI site found at 4641
    Illegal PstI site found at 5864
    Illegal PstI site found at 7416
    Illegal PstI site found at 7875
    Illegal NgoMIV site found at 2078
    Illegal NgoMIV site found at 2411
    Illegal NgoMIV site found at 4878
    Illegal NgoMIV site found at 5091
    Illegal NgoMIV site found at 5669
    Illegal NgoMIV site found at 6069
    Illegal NgoMIV site found at 6864
    Illegal AgeI site found at 1021
    Illegal AgeI site found at 3729
    Illegal AgeI site found at 4101
    Illegal AgeI site found at 4913
    Illegal AgeI site found at 6600
    Illegal AgeI site found at 6744
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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